• Based on the differentiation and siRNA

  2018-11-08

  

  Based on the differentiation and siRNA studies reported here, the mechanistic regulation of FOXM1 towards pluripotency control and differentiation might differ between mouse and human ES cells. The discrepancy can be attributed to the different developmental status of human versus mouse ES cells (Ni

• In contrast to bona fide ESC lines the pre

  2018-11-08

  

  In pka inhibitor to bona fide ESC lines, the pre-ESC lines expressed H19 and IGF2 mRNAs and lower levels of DNMT3B. This may reflect their limited ability to differentiate into the three germ layers, as a high H19 expression level has been associated with altered differentiation potential in parthe

• Urine of the patient was collected

  2018-11-08

  

  Urine of the patient was collected for harmine hydrochloride isolation and expansion. The urine cells were tested for free of mycoplasma. For reprogramming, the urine cells were transfected with episomal plasmids expressing Oct4, Sox2, Klf4 and miR-302-367 cluster. About 3weeks after transfection,

• Pathway analysis on these genes

  2018-11-08

  

  Pathway analysis on these genes identified their role in stem-cell-maintenance-related Wnt pathway (Baker, 2010), cell-cycle-related p53 pathway, JAK stat, and MAPK pathways in addition to others listed in Table S4. These results suggest a potential contribution of multiple signaling pathways for st

• br Conclusions Whilst it is clear

  2018-11-08

  

  Conclusions Whilst it is clear that RNA-binding proteins play crucial roles in regulating oogenesis, understanding the functions of these proteins relies on identifying the RNA targets they bind and regulate. Given the relative promiscuity of RNA-binding proteins, it seems likely that the fertil

• br Materials and methods br Acknowledgement This

  2018-11-08

  

  Materials and methods Acknowledgement This work was supported by the Korea National Institute of Health (grant number 2016-NG61002-00, 2016). Resource table Resource details E15.5 embryos were obtained from the crossing of heterozygous Sprague-Dawley (SD)-Tg(GFP)1BalRrrc rats. Rat embryos

• Mechanisms underlying CIPN include direct and indirect effec

  2018-11-08

  

  Mechanisms underlying CIPN include direct and indirect effects on sensory nerves such as damage to neuronal cell bodies in the dorsal root ganglion, alteration of the amplitude of the apoptosis or conduction velocity (Argyriou et al., 2012; Sisignano et al., 2014). Whereas CIPN may be reversible fo

• br Experimental Procedures br Author Contributions M A plann

  2018-11-08

  

  Experimental Procedures Author Contributions M.A. planned and performed the experiments and wrote the manuscript. T.N. helped in performing CTL redifferentiation experiments. S.Y. provided scientific discussion and technical support. T.Y. provided plasmids and helped in generating lentivirus v

• daminozide We observed no off target mutations in

  2018-11-08

  

  We observed no off-target mutations in our edited cell lines at eight bioinformatically predicted sites by using one-pot sgRNAs, although we cannot rule out the presence of mutations at other loci. Because optimized Cas9 mRNA and protein delivery can reduce off-target mutagenesis (Kim et al., 2014),

• br Experimental Procedures br Author Contributions br Acknow

  2018-11-07

  

  Experimental Procedures Author Contributions Acknowledgments We thank Toshio Kitamura for PLAT-A cells, and Gregg Duester and Daniel J. Donoghue for discussions. Yoav Altman, Joseph Russo, Edward Monosov, Frederick Lo, Maria Jose Martinez Lopez, Justin D. Blethrow, and Alexey Eroshkin kindl

• Go 6976 Previous reports have described ESC

  2018-11-07

  

  Previous reports have described ESC-based differentiation in collagen gels to study the developmental events of vasculogenesis and angiogenesis (Feraud et al., 2001; Hermant et al., 2007). However, these were not optimized for the assessment of more fundamental aspects of vessel induction, patternin

• br Discussion Child health research has largely been informe

  2018-11-07

  

  Discussion Child health research has largely been informed by traditional models of child development in which children progress through predictable and universal stages of development (McIntosh et al., 2013, p. 4). Within this framing, didactic teaching of knowable facts is prioritised and child

• br Introduction Thorstein Veblen s deals

  2018-11-07

  

  Introduction Thorstein Veblen\'s deals with the psychological, social, and economic issues of how institutions influence people\'s behavior from an evolutionary perspective. For Veblen, neither individuals nor institutions are taken for granted; he analyzes each from the beginning of their existe

• The consequences of the reduced exchange

  2018-11-07

  

  The consequences of the reduced exchange rate effectiveness may be observed when we make a simulation exercise based on the estimated coefficients from unrestricted model described in the column 11 of Table 3. These estimated coefficients have been chosen given that it is the SYS-GMM estimation cons

• It is clear that Lucas s insight was not new

  2018-11-07

  

  It is clear that Lucas\'s insight was not new, he even suggest its existence on the works of Frank Knight, for instance. Hicks (1936: 241) in his review of the Keynes\'s General Theory, for example, affirmed: “It is unrealistic to assume that an important change in data – say the introduction or ext

15697 records 1029/1047 page Previous Next First page 上5页 10261027102810291030 下5页 Last page