• br Conflict of interest statement The following are the

  2018-11-08

  

  Conflict of interest statement The following are the supplementary data related to this article. Acknowledgments This research was supported by funds to R.C. and F.C. from the Italian Ministry of Education, University and Research (MIUR). Introduction Besides hematopoietic stem leukotrien

• br H AZ histone exchange H AZ is incorporated within

  2018-11-08

  

  H2AZ histone exchange H2AZ is incorporated within nucleosomes via ATP-dependent activities. The complexes mediating H2AZ-H2A exchanges are well characterized in Saccharomyces cerevisiae and include the Swr1-containing SWR complex (SWR-C). The mammalian orthologue of Swr1 is SRCAP. Both complexes

• aminopeptidase Early studies demonstrated that primitive cel

  2018-11-08

  

  Early studies demonstrated that primitive aminopeptidase are readily converted into iPSCs (Kim et al., 2008; Kleger et al., 2012; Tsai et al., 2011). Thus, we expected that Lgr5+ cells would be susceptible to reprogramming. Unexpectedly, Lgr5+ cells from HFs and MEFs showed more resistance to early

• From an analytical perspective we

  2018-11-08

  

  From an analytical perspective, we applied the approach that combines application of high pressure assisted protein extraction and a combination of two LC/MS/MS techniques: electrospray ionization (ESI)-MSe and MALDI-TOF/TOF (Mindaye et al., 2013a, 2013b). Label-free quantification of proteins was p

• Although it is possible that reduced

  2018-11-08

  

  Although it is possible that reduced shear stresses in the injured colon may have favoured HPC7 adhesion, this study suggests that active adhesive mechanisms, similar to those utilised by inflammatory neutrophils, were actually involved in mediating their recruitment. There has been much recent disc

• Based on the differentiation and siRNA

  2018-11-08

  

  Based on the differentiation and siRNA studies reported here, the mechanistic regulation of FOXM1 towards pluripotency control and differentiation might differ between mouse and human ES cells. The discrepancy can be attributed to the different developmental status of human versus mouse ES cells (Ni

• In contrast to bona fide ESC lines the pre

  2018-11-08

  

  In pka inhibitor to bona fide ESC lines, the pre-ESC lines expressed H19 and IGF2 mRNAs and lower levels of DNMT3B. This may reflect their limited ability to differentiate into the three germ layers, as a high H19 expression level has been associated with altered differentiation potential in parthe

• Urine of the patient was collected

  2018-11-08

  

  Urine of the patient was collected for harmine hydrochloride isolation and expansion. The urine cells were tested for free of mycoplasma. For reprogramming, the urine cells were transfected with episomal plasmids expressing Oct4, Sox2, Klf4 and miR-302-367 cluster. About 3weeks after transfection,

• Pathway analysis on these genes

  2018-11-08

  

  Pathway analysis on these genes identified their role in stem-cell-maintenance-related Wnt pathway (Baker, 2010), cell-cycle-related p53 pathway, JAK stat, and MAPK pathways in addition to others listed in Table S4. These results suggest a potential contribution of multiple signaling pathways for st

• br Conclusions Whilst it is clear

  2018-11-08

  

  Conclusions Whilst it is clear that RNA-binding proteins play crucial roles in regulating oogenesis, understanding the functions of these proteins relies on identifying the RNA targets they bind and regulate. Given the relative promiscuity of RNA-binding proteins, it seems likely that the fertil

• br Materials and methods br Acknowledgement This

  2018-11-08

  

  Materials and methods Acknowledgement This work was supported by the Korea National Institute of Health (grant number 2016-NG61002-00, 2016). Resource table Resource details E15.5 embryos were obtained from the crossing of heterozygous Sprague-Dawley (SD)-Tg(GFP)1BalRrrc rats. Rat embryos

• Mechanisms underlying CIPN include direct and indirect effec

  2018-11-08

  

  Mechanisms underlying CIPN include direct and indirect effects on sensory nerves such as damage to neuronal cell bodies in the dorsal root ganglion, alteration of the amplitude of the apoptosis or conduction velocity (Argyriou et al., 2012; Sisignano et al., 2014). Whereas CIPN may be reversible fo

• br Experimental Procedures br Author Contributions M A plann

  2018-11-08

  

  Experimental Procedures Author Contributions M.A. planned and performed the experiments and wrote the manuscript. T.N. helped in performing CTL redifferentiation experiments. S.Y. provided scientific discussion and technical support. T.Y. provided plasmids and helped in generating lentivirus v

• daminozide We observed no off target mutations in

  2018-11-08

  

  We observed no off-target mutations in our edited cell lines at eight bioinformatically predicted sites by using one-pot sgRNAs, although we cannot rule out the presence of mutations at other loci. Because optimized Cas9 mRNA and protein delivery can reduce off-target mutagenesis (Kim et al., 2014),

• br Experimental Procedures br Author Contributions br Acknow

  2018-11-07

  

  Experimental Procedures Author Contributions Acknowledgments We thank Toshio Kitamura for PLAT-A cells, and Gregg Duester and Daniel J. Donoghue for discussions. Yoav Altman, Joseph Russo, Edward Monosov, Frederick Lo, Maria Jose Martinez Lopez, Justin D. Blethrow, and Alexey Eroshkin kindl

15582 records 1021/1039 page Previous Next First page 上5页 10211022102310241025 下5页 Last page